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Shining a spotlight on polyploid cells: New tool uncovers spatial patterns of DNA content across tissues
A collaborative effort by the Formosa-Jordan lab from the Max Planck Institute for Plant Breeding Research in Cologne, Germany, the Fox lab from Duke University, U.S., and the Roeder lab from Cornell ...
Scientists have long known that some cells in a given tissue undergo a process of duplicating their entire genome without dividing – a process called endopolyploidy, where cellular ploidy is the copy ...
Confocal microscopy is performed with nuclear reporters and stains, nuclear segmentation with ilastik is conducted, and a machine learning method is used to classify the ploidy of each nucleus. Colors ...
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