Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform at reduced pH 1. This method allows many samples to be processed ...

The "Extraction buffer" helps in carrying ... can be used to remove these contaminants, leaving the DNA in a pure form. The standard way to de-proteinize a cell extract is to add phenol or a 1:1 ...