The genes lacZ, lacY and lacA are transcribed from a single promoter (P) that produces a single mRNA from which the three proteins are translated. The operon is regulated by Lac repressor ...

1). In the 'off' state, the Lac inhibitor represses the lac operon, which prevents expression of the Tet repressor and the target gene. Additionally, a short hairpin RNA (shRNA) against the target ...